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KMID : 1134820220510090886
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Journal of the Korean Society of Food Science and Nutrition
2022 Volume.51 No. 9 p.886 ~ p.893
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The In Vitro and In Vivo Inhibitory Effect of Combined Extracts of Cervus elaphus Antler, Glycyrrhiza uralensis, and Angelica gigas on Benign Prostatic Hyperplasia
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Lee Jeong-Yoon
Son Hyung-Gu
Kim So-Hyuk
Lee Ki-Won
Kim Jin-Soo
Kim In-Hwan
Koo Young-Tae
Lee Yoo-Hyun
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Abstract
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Combinations of natural products have a number of benefits. We investigated the inhibitory effect of a combination of 50% ethanol extracts of Cervus elaphus antler (CEE), Glycyrrhiza uralensis (GUE), and Angelica gigas (AGE) on benign prostatic hyperplasia (BPH) using BPH-1 cells and testosterone propionate (TP)-induced BPH rats. The combination, called CGA113, inhibited cell proliferation by decreasing the cell viability of BPH-1 cells up to 72.03% more than the individual extracts. In addition, CGA113 significantly reduced the mRNA expressions of AR and SRD5A2 in BPH-1 cells. Sprague-Dawley rats were subjected to castration surgery to remove endogenous testosterone, and 3 mg/kg testosterone propionate was subcutaneously injected to induce BPH. Subsequently, CGA113 (50 and 200 mg/kg BW/day) was orally administered to the TP-induced BPH rats for 8 weeks. In the CGA113-H group (200 mg/kg of CGA113), the prostate ratio, serum DHT, prostate DHT, and prostate 5α-reductase2 were significantly decreased compared to the BPH group. Histology with H&E staining of the prostate tissue, revealed a reduction in the thickening of the prostate epithelial cells and enlargement of the lumen compared to the BPH group. The mRNA levels of the Bax/Bcl2 ratio in prostate tissue of the CGA113-H group were significantly increased. Overall, the combined extract of CEE, GUE, and AGE, was determined to exert synergistic effects, indicating the potential of the three natural materials as therapeutic agents for alleviation of BPH symptoms.
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KEYWORD
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androgen receptor, Angelica gigas, benign prostatic hyperplasia, Cervus elaphus antler, Glycyrrhiza uralensis
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